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ccne2  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology ccne2
    Ccne2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ccne2/cyclin+E2+Antibody/pm41431399-98-50-51
    Average 93 stars, based on 75 article reviews
    ccne2 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: High resolution profiling of cell cycle-dependent protein and phosphorylation abundance changes in non-transformed cells
    Article Snippet: .. After 1 h blocking with 5% milk in TBST at room temperature (RT) for 1 h, membranes were incubated overnight at 4 °C with the following primary antibodies: CCND1 (Santa Cruz Biotechnology, sc-20044, 1:500), CCND3 (Santa Cruz Biotechnology, sc-6283, 1:500), CCNE2 (Santa Cruz Biotechnology, sc-248, 1:500), CCNA (Santa Cruz Biotechnology, sc-271682, 1:500), CCNA (Santa Cruz Biotechnology, sc-596, 1:500), CCNB1 (Santa Cruz Biotechnology, sc-245, 1:500), GAPDH (Santa Cruz Biotechnology, sc-51907, 1:500), CDK1 pT161 (Cell Signalling Technology, 9114, 1:1000), CDK1 pY15 (Cell Signalling Technology, 9111, 1:1000), Alpha Tubulin (Abcam, ab52866, 1:5000), PLK1(Millipore, 05-844, 1:1000), CDK2 (Santa Cruz Biotechnology, sc-163, 1:500), CDK1 (Abcam, ab32094, 1:1000), CDC20 (Santa Cruz Biotechnology, sc-13162, 1:500), UBR7 (Thermo Fisher Scientific, A304-130A, 1:1000), UBE2S (Cell Signalling Technology, 11878, 1:1000), CDKN1B (Dako, M7203, 1:1000), CDKN1B pS10 (Santa Cruz Biotechnology, sc-12939, 1:500), NCL (Abcam, ab22758, 1:2000), NCL pT76 (Abcam, ab168363, 1:1000), CCNB1 (Santa Cruz Biotechnology, sc-245, 1:500), SGO2 (Thermo Fisher Scientific, A301-261A, 1:1000), GTSE1 (Abnova, H00051512-B01P, 1:1000), MELK (Thermo Fisher Scientific, A303-136A, 1:1000), FAM83D (Custom antibody from Erich Nigg, 1:500), ANAPC4 (Thermo Fisher Scientific, A301-175A, 1:1000), Phospho-CDK substrate motif [(K/H)pSP] (Cell Signalling Technology, 9477, 1:1000), JUNB (Proteintech, 10486-1-AP, 1:1000), CHAF1B (Thermo Fisher Scientific, A301-085A, 1:2000), Histone H3 pS10 (Cell Signalling Technology, 3377, 1:1000), Actin (Sigma-Aldrich, A5441, 1:5000). .. After washed with TBST for three times, membranes were incubated for 1 h at RT with the following secondary antibodies: IRDye 680RD Donkey anti-Rabbit IgG (H + L) (LI-COR Biosciences, 926-68073, 1:10000), IRDye 800CW Donkey anti-Rabbit IgG (H + L) (LI-COR Biosciences, 926-32213, 1:10000), IRDye 800CW Donkey anti-Mouse IgG (H + L) (LI-COR Biosciences, 926-32212, 1:10000), IRDye 680RD Donkey anti-Rabbit IgG (H + L) (LI-COR Biosciences, 926-68073, 1:10000), anti-mouse HRP (Cell Signalling Technology, 7076, 1:2000), anti-rabbit HRP (Cell Signalling Technology, 7074, 1:2000).

    Article Title: High resolution profiling of cell cycle-dependent protein and phosphorylation abundance changes in non-transformed cells
    Article Snippet: .. After 1 h blocking with 5% milk in TBST at room temperature (RT) for 1 h, membranes were incubated overnight at 4°C with the following primary antibodies: CCND1 (Santa Cruz Biotechnology, sc-2004), CCND3 (Santa Cruz Biotechnology, sc-6283), CCNE2 (Santa Cruz Biotechnology, sc-248), CCNA (Santa Cruz Biotechnology, sc-271682), CCNA (Santa Cruz Biotechnology, sc-596), CCNB1 (Santa Cruz Biotechnology, sc-245), GAPDH (Santa Cruz Biotechnology, sc-51907), CDK1 pT161 (Cell Signalling Technology, 9114), CDK1 pY15 (Cell Signalling Technology, 9111), Alpha Tubulin (Abcam, ab52866), PLK1(Millipore, 05-844), CDK2 (Santa Cruz Biotechnology, sc-163), CDK1 (Abcam, ab32094), CDC20 (Santa Cruz Biotechnology, sc-13162), UBR7 (Thermo Fisher Scientific, A304-130A), UBE2S (Cell Signalling Technology, 11878), CDKN1B (Dako, M7203), CDKN1B pS10 (Santa Cruz Biotechnology, sc-12939), NCL (Abcam, ab22758), NCL pT76 (Abcam, ab168363), CCNB1 (Santa Cruz Biotechnology, sc-245), SGO2 (Thermo Fisher Scientific, A301-261A), GTSE1 (Abnova, H00051512-B01P), MELK (Thermo Fisher Scientific, A303-136A), FAM83D (Custom antibody from Erich Nigg), ANAPC4 (Thermo Fisher Scientific, A301-175A), Phospho-CDK substrate motif [(K/H)pSP] (Cell Signalling Technology, 9477), JUNB (Proteintech, 10486-1-AP), CHAF1B (Thermo Fisher Scientific, A301-085A), Histone H3 pS10 (Cell Signalling Technology, 3377), Actin (Sigma-Aldrich, A5441). .. After washed with TBST for three times, membranes were incubated for 1h at RT with the following secondary antibodies: IRDye 680RD Donkey anti-Rabbit IgG (H+L) (LI-COR Biosciences, 926-68073), IRDye 800CW Donkey anti-Rabbit IgG (H + L) (LI-COR Biosciences, 926-32213), IRDye 800CW Donkey anti-Mouse IgG (H + L) (LI-COR Biosciences, 926-32212), IRDye 680RD Donkey anti-Rabbit IgG (H + L) (LI-COR Biosciences, 926-68073), anti-mouse HRP (Cell Signalling Technology, 7076), anti-rabbit HRP (Cell Signalling Technology, 7074).

    Article Title: High resolution profiling of cell cycle-dependent protein and phosphorylation abundance changes in non-transformed cells.
    Article Snippet: .. After 1 h blocking with 5% milk in TBST at room temperature (RT) for 1 h, membranes were incubated overnight at 4 °C with the following primary antibodies: CCND1 (Santa Cruz Biotechnology, sc-20044, 1:500), CCND3 (Santa Cruz Biotechnology, sc-6283, 1:500), CCNE2 (Santa Cruz Biotechnology, sc-248, 1:500), CCNA (Santa Cruz Biotechnology, sc-271682, 1:500), CCNA (Santa Cruz Biotechnology, sc-596, 1:500), CCNB1 (Santa Cruz Biotechnology, sc-245, 1:500), GAPDH (Santa Cruz Biotechnology, sc-51907, 1:500), CDK1 pT161 (Cell Signalling Technology, 9114, 1:1000), CDK1 pY15 (Cell Signalling Technology, 9111, 1:1000), Alpha Tubulin (Abcam, ab52866, 1:5000), PLK1(Millipore, 05-844, 1:1000), CDK2 (Santa Cruz Biotechnology, sc-163, 1:500), CDK1 (Abcam, ab32094, 1:1000), CDC20 (Santa Cruz Biotechnology, sc-13162, 1:500), UBR7 (Thermo Fisher Scientific, A304-130A, 1:1000), UBE2S (Cell Signalling Technology, 11878, 1:1000), CDKN1B (Dako, M7203, 1:1000), CDKN1B pS10 (Santa Cruz Biotechnology, sc-12939, 1:500), NCL (Abcam, ab22758, 1:2000), NCL pT76 (Abcam, ab168363, 1:1000), CCNB1 (Santa Cruz Biotechnology, sc-245, 1:500), SGO2 (Thermo Fisher Scientific,A301-261A, 1:1000), GTSE1 (Abnova, H00051512-B01P, 1:1000), MELK (Thermo Fisher Scientific, A303-136A, 1:1000), FAM83D (Custom antibody from Erich Nigg, 1:500), ANAPC4 (Thermo Fisher Scientific, A301-175A, 1:1000), Phospho-CDK substrate motif [(K/H) pSP] (Cell Signalling Technology, 9477, 1:1000), JUNB (Proteintech, 10486-1-AP, 1:1000), CHAF1B (Thermo Fisher Scientific, A301-085A, 1:2000), Histone H3 pS10 (Cell Signalling Technology, 3377, 1:1000), Actin (Sigma-Aldrich, A5441, 1:5000). .. Afterwashedwith TBST for three times, membranes were incubated for 1 h at RT with the following secondary antibodies: IRDye 680RDDonkey anti-Rabbit IgG (H + L) (LICOR Biosciences, 926-68073, 1:10000), IRDye 800CW Donkey antiRabbit IgG (H+ L) (LI-COR Biosciences, 926-32213, 1:10000), IRDye 800CW Donkey anti-Mouse IgG (H+ L) (LI-COR Biosciences, 926- 32212, 1:10000), IRDye 680RD Donkey anti-Rabbit IgG (H + L) (LI-COR Biosciences, 926-68073, 1:10000), anti-mouse HRP (Cell Signalling Technology, 7076, 1:2000), anti-rabbit HRP (Cell Signalling Technology, 7074, 1:2000).

    Incubation:

    Article Title: High resolution profiling of cell cycle-dependent protein and phosphorylation abundance changes in non-transformed cells
    Article Snippet: .. After 1 h blocking with 5% milk in TBST at room temperature (RT) for 1 h, membranes were incubated overnight at 4 °C with the following primary antibodies: CCND1 (Santa Cruz Biotechnology, sc-20044, 1:500), CCND3 (Santa Cruz Biotechnology, sc-6283, 1:500), CCNE2 (Santa Cruz Biotechnology, sc-248, 1:500), CCNA (Santa Cruz Biotechnology, sc-271682, 1:500), CCNA (Santa Cruz Biotechnology, sc-596, 1:500), CCNB1 (Santa Cruz Biotechnology, sc-245, 1:500), GAPDH (Santa Cruz Biotechnology, sc-51907, 1:500), CDK1 pT161 (Cell Signalling Technology, 9114, 1:1000), CDK1 pY15 (Cell Signalling Technology, 9111, 1:1000), Alpha Tubulin (Abcam, ab52866, 1:5000), PLK1(Millipore, 05-844, 1:1000), CDK2 (Santa Cruz Biotechnology, sc-163, 1:500), CDK1 (Abcam, ab32094, 1:1000), CDC20 (Santa Cruz Biotechnology, sc-13162, 1:500), UBR7 (Thermo Fisher Scientific, A304-130A, 1:1000), UBE2S (Cell Signalling Technology, 11878, 1:1000), CDKN1B (Dako, M7203, 1:1000), CDKN1B pS10 (Santa Cruz Biotechnology, sc-12939, 1:500), NCL (Abcam, ab22758, 1:2000), NCL pT76 (Abcam, ab168363, 1:1000), CCNB1 (Santa Cruz Biotechnology, sc-245, 1:500), SGO2 (Thermo Fisher Scientific, A301-261A, 1:1000), GTSE1 (Abnova, H00051512-B01P, 1:1000), MELK (Thermo Fisher Scientific, A303-136A, 1:1000), FAM83D (Custom antibody from Erich Nigg, 1:500), ANAPC4 (Thermo Fisher Scientific, A301-175A, 1:1000), Phospho-CDK substrate motif [(K/H)pSP] (Cell Signalling Technology, 9477, 1:1000), JUNB (Proteintech, 10486-1-AP, 1:1000), CHAF1B (Thermo Fisher Scientific, A301-085A, 1:2000), Histone H3 pS10 (Cell Signalling Technology, 3377, 1:1000), Actin (Sigma-Aldrich, A5441, 1:5000). .. After washed with TBST for three times, membranes were incubated for 1 h at RT with the following secondary antibodies: IRDye 680RD Donkey anti-Rabbit IgG (H + L) (LI-COR Biosciences, 926-68073, 1:10000), IRDye 800CW Donkey anti-Rabbit IgG (H + L) (LI-COR Biosciences, 926-32213, 1:10000), IRDye 800CW Donkey anti-Mouse IgG (H + L) (LI-COR Biosciences, 926-32212, 1:10000), IRDye 680RD Donkey anti-Rabbit IgG (H + L) (LI-COR Biosciences, 926-68073, 1:10000), anti-mouse HRP (Cell Signalling Technology, 7076, 1:2000), anti-rabbit HRP (Cell Signalling Technology, 7074, 1:2000).

    Article Title: High resolution profiling of cell cycle-dependent protein and phosphorylation abundance changes in non-transformed cells
    Article Snippet: .. After 1 h blocking with 5% milk in TBST at room temperature (RT) for 1 h, membranes were incubated overnight at 4°C with the following primary antibodies: CCND1 (Santa Cruz Biotechnology, sc-2004), CCND3 (Santa Cruz Biotechnology, sc-6283), CCNE2 (Santa Cruz Biotechnology, sc-248), CCNA (Santa Cruz Biotechnology, sc-271682), CCNA (Santa Cruz Biotechnology, sc-596), CCNB1 (Santa Cruz Biotechnology, sc-245), GAPDH (Santa Cruz Biotechnology, sc-51907), CDK1 pT161 (Cell Signalling Technology, 9114), CDK1 pY15 (Cell Signalling Technology, 9111), Alpha Tubulin (Abcam, ab52866), PLK1(Millipore, 05-844), CDK2 (Santa Cruz Biotechnology, sc-163), CDK1 (Abcam, ab32094), CDC20 (Santa Cruz Biotechnology, sc-13162), UBR7 (Thermo Fisher Scientific, A304-130A), UBE2S (Cell Signalling Technology, 11878), CDKN1B (Dako, M7203), CDKN1B pS10 (Santa Cruz Biotechnology, sc-12939), NCL (Abcam, ab22758), NCL pT76 (Abcam, ab168363), CCNB1 (Santa Cruz Biotechnology, sc-245), SGO2 (Thermo Fisher Scientific, A301-261A), GTSE1 (Abnova, H00051512-B01P), MELK (Thermo Fisher Scientific, A303-136A), FAM83D (Custom antibody from Erich Nigg), ANAPC4 (Thermo Fisher Scientific, A301-175A), Phospho-CDK substrate motif [(K/H)pSP] (Cell Signalling Technology, 9477), JUNB (Proteintech, 10486-1-AP), CHAF1B (Thermo Fisher Scientific, A301-085A), Histone H3 pS10 (Cell Signalling Technology, 3377), Actin (Sigma-Aldrich, A5441). .. After washed with TBST for three times, membranes were incubated for 1h at RT with the following secondary antibodies: IRDye 680RD Donkey anti-Rabbit IgG (H+L) (LI-COR Biosciences, 926-68073), IRDye 800CW Donkey anti-Rabbit IgG (H + L) (LI-COR Biosciences, 926-32213), IRDye 800CW Donkey anti-Mouse IgG (H + L) (LI-COR Biosciences, 926-32212), IRDye 680RD Donkey anti-Rabbit IgG (H + L) (LI-COR Biosciences, 926-68073), anti-mouse HRP (Cell Signalling Technology, 7076), anti-rabbit HRP (Cell Signalling Technology, 7074).

    Article Title: High resolution profiling of cell cycle-dependent protein and phosphorylation abundance changes in non-transformed cells.
    Article Snippet: .. After 1 h blocking with 5% milk in TBST at room temperature (RT) for 1 h, membranes were incubated overnight at 4 °C with the following primary antibodies: CCND1 (Santa Cruz Biotechnology, sc-20044, 1:500), CCND3 (Santa Cruz Biotechnology, sc-6283, 1:500), CCNE2 (Santa Cruz Biotechnology, sc-248, 1:500), CCNA (Santa Cruz Biotechnology, sc-271682, 1:500), CCNA (Santa Cruz Biotechnology, sc-596, 1:500), CCNB1 (Santa Cruz Biotechnology, sc-245, 1:500), GAPDH (Santa Cruz Biotechnology, sc-51907, 1:500), CDK1 pT161 (Cell Signalling Technology, 9114, 1:1000), CDK1 pY15 (Cell Signalling Technology, 9111, 1:1000), Alpha Tubulin (Abcam, ab52866, 1:5000), PLK1(Millipore, 05-844, 1:1000), CDK2 (Santa Cruz Biotechnology, sc-163, 1:500), CDK1 (Abcam, ab32094, 1:1000), CDC20 (Santa Cruz Biotechnology, sc-13162, 1:500), UBR7 (Thermo Fisher Scientific, A304-130A, 1:1000), UBE2S (Cell Signalling Technology, 11878, 1:1000), CDKN1B (Dako, M7203, 1:1000), CDKN1B pS10 (Santa Cruz Biotechnology, sc-12939, 1:500), NCL (Abcam, ab22758, 1:2000), NCL pT76 (Abcam, ab168363, 1:1000), CCNB1 (Santa Cruz Biotechnology, sc-245, 1:500), SGO2 (Thermo Fisher Scientific,A301-261A, 1:1000), GTSE1 (Abnova, H00051512-B01P, 1:1000), MELK (Thermo Fisher Scientific, A303-136A, 1:1000), FAM83D (Custom antibody from Erich Nigg, 1:500), ANAPC4 (Thermo Fisher Scientific, A301-175A, 1:1000), Phospho-CDK substrate motif [(K/H) pSP] (Cell Signalling Technology, 9477, 1:1000), JUNB (Proteintech, 10486-1-AP, 1:1000), CHAF1B (Thermo Fisher Scientific, A301-085A, 1:2000), Histone H3 pS10 (Cell Signalling Technology, 3377, 1:1000), Actin (Sigma-Aldrich, A5441, 1:5000). .. Afterwashedwith TBST for three times, membranes were incubated for 1 h at RT with the following secondary antibodies: IRDye 680RDDonkey anti-Rabbit IgG (H + L) (LICOR Biosciences, 926-68073, 1:10000), IRDye 800CW Donkey antiRabbit IgG (H+ L) (LI-COR Biosciences, 926-32213, 1:10000), IRDye 800CW Donkey anti-Mouse IgG (H+ L) (LI-COR Biosciences, 926- 32212, 1:10000), IRDye 680RD Donkey anti-Rabbit IgG (H + L) (LI-COR Biosciences, 926-68073, 1:10000), anti-mouse HRP (Cell Signalling Technology, 7076, 1:2000), anti-rabbit HRP (Cell Signalling Technology, 7074, 1:2000).

    Electrophoresis:

    Article Title: Interaction between ZMIZ2 and AR promotes prostate cancer proliferation in vitro and in vivo.
    Article Snippet: .. After electrophoresis, protein was transferred to PVDF and sealed with 5% milk for 1 h. Add following primary antibodies and incubate overnight: ZMIZ2 (Novus Biologicals, LLC, Centennial, CO, USA), AR (Santa Cruz Biotechnology, Dallas, TX, USA), CDK1 (Santa Cruz Biotechnology, Dallas, TX, USA), CCNA2 (Santa Cruz Biotechnology, Dallas, TX, USA), CCNE2 (Santa Cruz Biotechnology, Dallas, TX, USA). .. The protein blots were visualized by employing either a goat anti-rabbit IgG HRPconjugated secondary antibody or a goat anti-mouse IgG HRP-conjugated secondary antibody (purchased from CST, located in Danvers, Massachusetts, USA).

    Article Title: Interaction between ZMIZ2 and AR promotes prostate cancer proliferation in vitro and in vivo
    Article Snippet: .. After electrophoresis, protein was transferred to PVDF and sealed with 5% milk for 1 h. Add following primary antibodies and incubate overnight: ZMIZ2 (Novus Biologicals, LLC, Centennial, CO, USA), AR (Santa Cruz Biotechnology, Dallas, TX, USA), CDK1 (Santa Cruz Biotechnology, Dallas, TX, USA), CCNA2 (Santa Cruz Biotechnology, Dallas, TX, USA), CCNE2 (Santa Cruz Biotechnology, Dallas, TX, USA). .. The protein blots were visualized by employing either a goat anti-rabbit IgG HRP-conjugated secondary antibody or a goat anti-mouse IgG HRP-conjugated secondary antibody (purchased from CST, located in Danvers, Massachusetts, USA).

    Western Blot:

    Article Title: A patient-derived xenograft mouse platform from epithelioid glioblastoma provides possible druggable screening and translational study
    Article Snippet: .. The primary antibodies used in Western blotting and IHC included IDH1 R132H (H09): Dianova, Germany (1:100), Rb: #9313, Cell Signaling Technology, USA (1:1000), p-JNK: #4668, Cell Signaling Technology, USA (1:1000), JNK: #9252, Cell Signaling Technology, USA (1:1000), pRb: #9307, Cell Signaling Technology, USA (1:1000), ERK: SC1647, Santa Cruz Biotechnology, USA (1:500), p-ERK: SC-7383, Santa Cruz Biotechnology, USA (1:500), CCND1: GTX61306, GeneTex, USA (1:1000), CCNE2: 11935-AP, Proteintech (1:1000), CDK4: SC-23896, Santa Cruz Biotechnology, USA (1:1000), CDK6: SC-7961, Santa Cruz Biotechnology, USA (1:1000), β-actin: GT5512, GeneTex, USA (1:10000), BRAF V600E, VE1 (Ventana Medical Systems, automated Ventana Benchmark ULTRA platform). ..

    Immunohistochemistry:

    Article Title: A patient-derived xenograft mouse platform from epithelioid glioblastoma provides possible druggable screening and translational study
    Article Snippet: .. The primary antibodies used in Western blotting and IHC included IDH1 R132H (H09): Dianova, Germany (1:100), Rb: #9313, Cell Signaling Technology, USA (1:1000), p-JNK: #4668, Cell Signaling Technology, USA (1:1000), JNK: #9252, Cell Signaling Technology, USA (1:1000), pRb: #9307, Cell Signaling Technology, USA (1:1000), ERK: SC1647, Santa Cruz Biotechnology, USA (1:500), p-ERK: SC-7383, Santa Cruz Biotechnology, USA (1:500), CCND1: GTX61306, GeneTex, USA (1:1000), CCNE2: 11935-AP, Proteintech (1:1000), CDK4: SC-23896, Santa Cruz Biotechnology, USA (1:1000), CDK6: SC-7961, Santa Cruz Biotechnology, USA (1:1000), β-actin: GT5512, GeneTex, USA (1:10000), BRAF V600E, VE1 (Ventana Medical Systems, automated Ventana Benchmark ULTRA platform). ..



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    To uncover the molecular regulatory network underlying the functions of ZMIZ2 and AR, RNA-seq analysis was meticulously performed to identify the common downstream target genes of these two factors. (a) RNA-seq analysis of differentially expressed genes after ZMIZ2 silencing or AR silencing. (b) Venn diagram analysis of genes commonly upregulated by ZMIZ2 and AR. (c) KEGG analysis of genes commonly upregulated by ZMIZ2 and AR. (d) GO analysis of genes commonly upregulated by ZMIZ2 and AR. (e) Heatmap of the expression levels of cell cycle-related genes after ZMIZ2 silencing or AR silencing. (f–g) A flow cytometry assay was employed to determine the cell cycle distribution. (h) QPCR was utilized to assess the mRNA transcriptional levels of cell cycle-related genes. (i) Western blot analysis was carried out to detect the protein expression levels of CDK1, CCNA2, and <t>CCNE2</t> in each sample group. Significant differences are indicated as follows: * p < 0.05, ** p < 0.01, and *** p < 0.001; ns indicates not significant; n = 3.
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    Image Search Results


    To uncover the molecular regulatory network underlying the functions of ZMIZ2 and AR, RNA-seq analysis was meticulously performed to identify the common downstream target genes of these two factors. (a) RNA-seq analysis of differentially expressed genes after ZMIZ2 silencing or AR silencing. (b) Venn diagram analysis of genes commonly upregulated by ZMIZ2 and AR. (c) KEGG analysis of genes commonly upregulated by ZMIZ2 and AR. (d) GO analysis of genes commonly upregulated by ZMIZ2 and AR. (e) Heatmap of the expression levels of cell cycle-related genes after ZMIZ2 silencing or AR silencing. (f–g) A flow cytometry assay was employed to determine the cell cycle distribution. (h) QPCR was utilized to assess the mRNA transcriptional levels of cell cycle-related genes. (i) Western blot analysis was carried out to detect the protein expression levels of CDK1, CCNA2, and CCNE2 in each sample group. Significant differences are indicated as follows: * p < 0.05, ** p < 0.01, and *** p < 0.001; ns indicates not significant; n = 3.

    Journal: Cancer Biology & Therapy

    Article Title: Interaction between ZMIZ2 and AR promotes prostate cancer proliferation in vitro and in vivo

    doi: 10.1080/15384047.2025.2604936

    Figure Lengend Snippet: To uncover the molecular regulatory network underlying the functions of ZMIZ2 and AR, RNA-seq analysis was meticulously performed to identify the common downstream target genes of these two factors. (a) RNA-seq analysis of differentially expressed genes after ZMIZ2 silencing or AR silencing. (b) Venn diagram analysis of genes commonly upregulated by ZMIZ2 and AR. (c) KEGG analysis of genes commonly upregulated by ZMIZ2 and AR. (d) GO analysis of genes commonly upregulated by ZMIZ2 and AR. (e) Heatmap of the expression levels of cell cycle-related genes after ZMIZ2 silencing or AR silencing. (f–g) A flow cytometry assay was employed to determine the cell cycle distribution. (h) QPCR was utilized to assess the mRNA transcriptional levels of cell cycle-related genes. (i) Western blot analysis was carried out to detect the protein expression levels of CDK1, CCNA2, and CCNE2 in each sample group. Significant differences are indicated as follows: * p < 0.05, ** p < 0.01, and *** p < 0.001; ns indicates not significant; n = 3.

    Article Snippet: The ZMIZ2 antibody (Novus Biologicals, LLC, Centennial, CO, USA), AR antibody (Santa Cruz Biotechnology, Dallas, TX, USA), CDK1 antibody (Santa Cruz Biotechnology, Dallas, TX, USA), CCNA2 antibody (Santa Cruz Biotechnology, Dallas, TX, USA), CCNE2 antibody (Santa Cruz Biotechnology, Dallas, TX, USA) were added and incubated overnight at 4 °C and then incubated with HRP-conjugated goat anti-rabbit IgG (Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C for 30 min.

    Techniques: RNA Sequencing, Expressing, Flow Cytometry, Western Blot

    Depletion of ZMIZ2 expression is associated with reduced AR enrichment on the promoters of downstream target genes, accompanied by a concurrent decrease in H3K27ac levels. (a) Flow chart of the ChIP experiment. (b) The binding sites of AR on the promoters of CDK1, CCNA2, and CCNE2. (c–h) ChIP analysis of AR enrichment on the CDK1, CCNA2, and CCNE2 promoters and H3K27ac levels. Significant differences are indicated as follows: * p < 0.05, ** p < 0.01, and *** p < 0.001; ns indicates not significant; n = 3.

    Journal: Cancer Biology & Therapy

    Article Title: Interaction between ZMIZ2 and AR promotes prostate cancer proliferation in vitro and in vivo

    doi: 10.1080/15384047.2025.2604936

    Figure Lengend Snippet: Depletion of ZMIZ2 expression is associated with reduced AR enrichment on the promoters of downstream target genes, accompanied by a concurrent decrease in H3K27ac levels. (a) Flow chart of the ChIP experiment. (b) The binding sites of AR on the promoters of CDK1, CCNA2, and CCNE2. (c–h) ChIP analysis of AR enrichment on the CDK1, CCNA2, and CCNE2 promoters and H3K27ac levels. Significant differences are indicated as follows: * p < 0.05, ** p < 0.01, and *** p < 0.001; ns indicates not significant; n = 3.

    Article Snippet: The ZMIZ2 antibody (Novus Biologicals, LLC, Centennial, CO, USA), AR antibody (Santa Cruz Biotechnology, Dallas, TX, USA), CDK1 antibody (Santa Cruz Biotechnology, Dallas, TX, USA), CCNA2 antibody (Santa Cruz Biotechnology, Dallas, TX, USA), CCNE2 antibody (Santa Cruz Biotechnology, Dallas, TX, USA) were added and incubated overnight at 4 °C and then incubated with HRP-conjugated goat anti-rabbit IgG (Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C for 30 min.

    Techniques: Expressing, Binding Assay